- Deutsche Bahn - Deutsche Bahn is the nationalised operator of railways in Germany and operates the majority of passenger and freight services in the country. It is featured in all 3 German routes.
- Rapid Transit - Rapid Transit features the 71 km S-Bahn Mitteldeutschland S2 line from Markkleeberg-Gaschwitz to Dessau HBF via Leipzig. Featured with the route is the DB BR 1442 in S-Bahn Mitteldeutschland livery.
- Ruhr Sieg Nord - Ruhr Sieg Nord features the 61 km route from Hagen HBF to Finnentrop, winding through the Lenne valley in North-Rhine Westphalia. The route features a variety of freight and passenger services, utilising the DB BR 143, DB BR 185 and DB BR 767.2.
- Main-Spessart Bahn - Main-Spessart Bahn features the 52 km route from Aschaffenburg HBF to Gemünden in Bavaria including the 5.4 km Spessartrampe, which has a maximum gradient of 2.1%. Also included are the DB BR 146, DB BR 185 and DB BR 766.2.